Rapid population analysis of Magnaporthe grisea by using rep-PCR and endogenous repetitive DNA sequences. Longman Scientific and Technical, Harlow, UK. Pages 185-192 in: Evolution of Crop Plants. Pyricularia grisea isolates causing gray leaf spot on perennial ryegrass ( Lolium perenne) in the United States: Relationship to P. The Lolium pathotype of Magnaporthe oryzae recovered from a single blasted wheat plant in the United States. Farman, M., Peterson, G., Chen, L., Starnes, J., Valent, B., Bachi, P., Murdock, L., Hershman, D., Pedley, K.Food and Agricultural Commodities Production. The mitochondrial nad5 gene of sugar beet ( Beta vulgaris) encoding a subunit of the respiratory NADH dehydrogenase. Ecke, W., Schmitz, U., and Michaelis, G.Occurrence of blast fungus ( Pyricularia grisea) on wheat and rye in the savanna region of central Brazil. Natural infection of barley by P yricularia grisea in Brazil. Food and Agriculture Organization of the United Nations (FAO) Google Scholar A standardized inoculation protocol to test wheat cultivars for reaction to head blast caused by Magnaporthe oryzae (Triticum pathotype). wheat cultivars to the Triticum Pathotype of Magnaporthe oryzae. Preliminary assessment of resistance among U.S. A multilocus gene genealogy concordant with host preference indicates segregation of a new species, Magnaporthe oryzae, from M. Origins of host-specific populations of the blast pathogen Magnaporthe oryzae in crop domestication with subsequent expansion of pandemic clones on rice and weeds of rice. H., Tharreau, D., Valent, B., Van Kim, P., Nottéghem, J. Development of a warning system for wheat blast caused by Pyricularia grisea. Devastating wheat fungus appears in Asia for the first time. Centro de Investigación Agrícola Tropical, Informe Tecnico, Proyecto de Investigacion Trigo, Santa Cruz de la Sierra, Bolivia. 1996:Pages 76-86 in: Identificación y zonificación de Pyricularia o brusone (Pyricularia oryzae) en el cutivo de trigo en el departamento de Santa Cruz. Factors affecting infectivity, spread and persistence of Pyricularia oryzae Cav. Anderson, A., Henry, B., and Tullis, E.oryzae Triticum field surveillance, as well as identifying nonwheat species that may serve as a reservoir or source of inoculum for nearby wheat fields. The mitochondrial NADH-dehydrogenase ( nad5) gene, an internal control for plant DNA, was multiplexed with PoT2 and MoT3 and showed results comparable with individual assays. oryzae Triticum, respectively, using a field DNA extraction kit and the portable Genie II system. oryzae Triticum-infected wheat seed and spikes and identified M. oryzae Triticum, respectively, and sensitivity to approximately 5 pg of DNA per reaction. PoT2- and MoT3-based assays showed high specificity for M. Sensitivity was assessed using 10-fold serial dilutions of M. Negative controls included 50 Fusarium spp. oryzae strains collected from infected wheat and other grasses and representing geographic and temporal variation. Loop-mediated isothermal amplification (LAMP) primers were designed to target the PoT2 and MoT3 loci, previously shown to be specific for M. Currently, no in-field diagnostic exists for M. Wheat blast symptoms are similar to Fusarium head scab and can cause confusion in the field. Wheat blast, caused by the Magnaporthe oryzae Triticum pathotype, is an economically important fungal disease of wheat.
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